![]() We report the synthesis of stable core/shell (1-20 monolayers) QD-DNA conjugates in which the end of the phosphorothiolated oligonucleotide (5-10 nucleotides) is "embedded" within the shell of the QD. However, achieving DNA-functionalized semiconductor quantum dots (QDs) that are robust enough to withstand precipitation at high temperature and ionic strength through simple attachment of modified DNA to the QD surface remains a challenge. N2 - The assembly and isolation of DNA oligonucleotide-functionalized gold nanoparticles (AuNPs) has become a well-developed technology that is based on the strong bonding interactions between gold and thiolated DNA. T1 - Robust DNA-functionalized core/shell quantum dots with fluorescent emission spanning from UV-vis to near-IR and compatible with DNA-directed self-assembly These reliable QD-DNA conjugates exhibit excellent chemical and photonic stability, colloidal stability over a wide pH range (4-12) and at high salt concentrations (>100 mM Na+ or Mg2+), bright fluorescence emission with quantum yields of up to 70%, and broad spectral tunability with emission ranging from the UV to the NIR (360-800 nm).", We report the synthesis of stable core/shell (1-20 monolayers) QD-DNA conjugates in which the end of the phosphorothiolated oligonucleotide (5-10 nucleotides) is within the shell of the QD. The HRTEM images revealed the narrow size distribution and in addition, L-cysteine capped quantum dots were found to be more biocompatible than mercaptopropionicĪcid capped quantum dots which would provide new opportunities for applications in bioimaging and biolabelling.Abstract = "The assembly and isolation of DNA oligonucleotide-functionalized gold nanoparticles (AuNPs) has become a well-developed technology that is based on the strong bonding interactions between gold and thiolated DNA. It is observed that there is a considerable increase in the photoluminescence intensity of L-cysteine capped quantum dots than MPA. XRD results demonstrated the formation of hexagonal wurtzite structure for L-cysteine and cubic zinc blende structure for mercaptopropionic acid capped CdSe/CdS/ZnS quantum dots. On the quantum dots has been confirmed from Fourier transform infrared spectroscopy (FTIR). The capping of L-cysteine and mercaptopropionic acid The structural, morphologicalĪnd optical properties of these quantum dots have been examined through X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM), UV-Visible absorption spectroscopy (UV-Vis) and Photoluminescence spectroscopy (PL). The biocompatibility of the assynthesized quantum dots have also been analyzed through the cytotoxicity study using MTT assay. ![]() This article emphasizes on the aqueous synthesis of water soluble CdSe/CdS/ZnSĬore/shell/shell quantum dots with L-cysteine and mercaptopropionic acid as capping agent and their observed properties have been compared. ![]() Quantum dots have now become the most important candidates and widely exploited as promising architectures for use as diagnostic and imaging agents in biomedicine and as semiconductors in the electronics industry.
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